A7510
Adenosine 5′-Triphosphatase from porcine cerebral cortex
lyophilized powder, ≥0.3 units/mg protein, pH 7.8
Sinonimo/i:
ATP phosphohydrolase, ATPase
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Origine biologica
Porcine cerebral cortex
Livello qualitativo
Descrizione
Sodium and Potassium-Activated
Forma fisica
lyophilized powder
Attività specifica
≥0.3 units/mg protein, pH 7.8
Composizione
Protein, 5.0-25.0% biuret
tecniche
inhibition assay: suitable
Temperatura di conservazione
−20°C
Informazioni sul gene
pig ... ATP1A1(397481) , ATP1B1(396898)
Applicazioni
ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity .
Azioni biochim/fisiol
This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na+/K+-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells .
This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA).
Definizione di unità
One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37 °C in the presence of Na+, K+, and Mg2+.
Stato fisico
Lyophilized powder containing 90% sucrose, 0.4% EDTA Na4 and 0.06% NaCl
Nota sulla preparazione
Sodium iodide extracted fraction of Nakao, et al., Biochem. Biophys. Res. Commun., 19, 755 (1965).
Inibitore
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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