A6410
Apirasi
ATPase ≥200 units/mg protein, lyophilized powder
Sinonimo/i:
Adenosina 5′-difosfatasi, Adenosina 5′-trifosfatasi
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About This Item
Prodotti consigliati
Tipo
Grade VI
Livello qualitativo
Forma fisica
lyophilized powder
ATPase activity
≥200 units/mg protein
secondary activity
≤15 % of base activity ADPase
PM
~49 kDa by gel filtration
Composizione
Protein, 30-60%
Solubilità
H2O: soluble 1.0 mg/mL
Attività estranea
Acid Phosphatase ≤2% of base activity
Temperatura di conservazione
−20°C
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Descrizione generale
Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.
Applicazioni
Apyrase from potatoes has been used:
- to deplete ATP
- to degrade endogenous extracellular ATP
- as a supplement in acid citrate dextrose solution to treat blood obtained from anesthetized mice
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
In diverse varietà di S. tuberosum si trovano almeno due isoenzimi: uno con un rapporto ATPasi/ADPasi elevato (∼10) e un altro con un rapporto basso (∼1).
Reazione: ATP → ADP+Pi → AMP+2Pi.
Reazione: ATP → ADP+Pi → AMP+2Pi.
Azioni biochim/fisiol
Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition. Apyrase has adenosine 5′-triphosphatase (ATPase) and adenosine 5′-diphosphatase (ADPase) activities.
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Confezionamento
Sold on the basis of ATPase units.
Altre note
This grade has an exceptionally high ATPase/ADPase ratio.
Definizione di unità
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
Stato fisico
Partially purified, lyophilized powder containing potassium succinate buffer salts.
Nota sulla preparazione
Derived from white potato
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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