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Key Documents

A6284

Sigma-Aldrich

Human IgG−Agarose

suspension

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Coniugato

agarose conjugate

Livello qualitativo

Forma fisica

suspension

Grado di funzionalizzazione

≥5 mg per mL

Matrice

Cross-linked 4% beaded agarose

Attivazione matrice

cyanogen bromide

Braccio spaziatore

1 atom

Temperatura di conservazione

2-8°C

Descrizione generale

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.

Applicazioni

IgG-Agarose is an immuno-adsorbent that can be used to affinity purify antibodies, remove species-specific cross-reacting antibodies or remove contaminating antibodies from an antiserum preparation. Typically, cross-reacting antibodies may be removed from an antiserum preparation using an equal volume of IgG-Agarose (resin volume). However, the resin to antiserum ratio will vary with individual applications.
Tandem affnitiy purification pulldown was performed in yeast cultures using agarose-linked human IgG. Supernatants were mixed with 30μl of agarose beads overnight.

Stato fisico

Suspension in 0.5 M NaCl containing preservative.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Graciela C Calabrese et al.
Thrombosis research, 105(6), 537-541 (2002-07-02)
Antithrombin (AT) high affinity of unfractionated heparin (UFH) resides in a specific pentasaccharide sequence. Heparin also regulates complement activity on the classical and the alternative pathways. Most experimental pieces of evidence accumulated show that these important activities reside in different
Pierre Fotso et al.
The Journal of biological chemistry, 280(30), 27613-27623 (2005-06-04)
The conserved oligomeric Golgi (COG) complex is an evolutionarily conserved peripheral membrane oligomeric protein complex that is involved in intra-Golgi protein trafficking. The COG complex is composed of eight subunits that are located in two lobes; Lobe A contains COG1-4
Graciela C Calabrese et al.
Thrombosis research, 113(3-4), 243-250 (2004-05-14)
Dermatan sulfate (DS) is a member of the family of structurally complex, sulfated, linear heteropolysaccharides called glycosaminoglycans (GAGs). It has a similar structure to heparin and heparan sulfate (HS), but with acetylgalactosamine replacing glucosamine, and the uronic acid moiety, mainly
A G Norden et al.
Clinical chemistry, 43(6 Pt 1), 957-962 (1997-06-01)
A novel interference with measurements of serum free thyroxine (FT4) caused by rheumatoid factor (RhF) is described. We found misleading, sometimes gross, increases of FT4 results in 5 clinically euthyroid elderly female patients with high RhF concentrations. All 5 patients
Yeon Jeong Kim et al.
Frontiers in plant science, 10, 667-667 (2019-06-14)
Control of protein turnover is a key post-translational control point in the oscillatory network of the circadian clock. Some elements, such as TOC1 and PRR5 are engaged by a well-described F-box protein, ZEITLUPE, dedicated to their proteolytic turnover to shape

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