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Key Documents

A3316

Sigma-Aldrich

Anti-Human IgG (Fc Specific)−Agarose antibody produced in goat

affinity isolated antibody, PBS suspension

Sinonimo/i:

Goat anti-human IgG

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

goat

Coniugato

agarose conjugate

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Forma fisica

PBS suspension

Reattività contro le specie

human

Non deve reagire con

rat, mouse

tecniche

Ouchterlony double diffusion: suitable

Capacità

2 mg/mL, resin binding capacity (human IgG)

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is specific for the Fc fragment of human IgG when tested against purified human IgA, IgG, IgM, Fc, and kappa and lambda light chains. No reactivity with mouse or rat IgG is seen by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling.

Immunogeno

Fc fragment of human IgG.

Applicazioni

Anti-Human IgG (Fc Specific)-Agarose antibody produced in goat has also been used to purify galanin 1 (Gala1), 3Gal-substituted P-selectin glycoprotein ligand-1 (PSGL-1)/hIgG1 from supernatants of transfected COS cells.
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is suitable for use in Ouchterlony double diffusion. The antibody has also been used for affinity purification assays.
Immunoprecipitation assays were performed using goat anti-human IgG (Fc specific) crosslinked to agarose beads. The beads were diluted in 1 ml of 5% FBS/DMEM for 2 hours at 4 degrees.

Altre note

Antibody adsorbed with mouse and rat IgG

Stato fisico

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3


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