H9658

Anticorpo monoclonale Anti-HA

clone HA-7, ascites fluid

• Sinonimo/i: Anti-HA, Anti-emoagglutinina dell’influenza
• Numero MDL: MFCD00803873
• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: ascites fluid
• Tipo di anticorpo: primary antibodies
• Clone: HA-7, monoclonal
• contiene: 15 mM sodium azide
• tecniche: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein
• Isotipo: IgG1
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

The gene encoding influenza virus hemagglutinin (HA) precursor is mapped on segment 4. It encodes a 76kDa surface glycoprotein which exists as trimers on the viral envelope.

Specificità

L′anticorpo riconosce le forme native come quelle denaturate-ridotte delle proteine marcate con HA ed è reattivo con le proteine di fusione marcate con HA N- o C-terminale in E. coli o in cellule di mammifero.

Immunogeno

peptide sintetico corrispondente a un frammento dell′emoagglutinina (HA) del virus dell′influenza umana noto come HA-tag, coniugato con KLH

Applicazioni

Monoclonal Anti-HA antibody produced in mouse has been used in 293T cells for:

• immunoprecipitation in human embryonic kidney 293 cells
• immunoblotting in HeLa cells
• immunopurification in human embryonic kidney 293 cells
• immunostaining for electron microscopy in mouse embryonic fibroblasts
• in immunocytochemistry of hippocampal slices

Monoclonal Anti-HA antibody produced in mouse has been used in:

• Immunoblotting, to study the expression of fusion protein
• Coimmunoprecipitation assay
• Immunoblotting of lysate protein

Azioni biochim/fisiol

Hemagglutinin (HA) is the surface glycoprotein of influenza virus. HA is crucial for virus entry and interaction with N-acetylneuraminic acid (Neu5Ac) in host cell surface. HA coordinates with neuraminidase (NA) and facilitates viral entry into host. HA exists as a trimer and each monomer has stalk transmembrane and globular regions. The globular region contains carbohydrate side chains crucial for infecting host.

Influenza virus hemagglutinin (HA) acts as a key immunogen in avian influenza virus (AIV) vaccines. Activated HA facilitates virus attachment to oligosaccharide receptors on host cell surface and is involved in penetration through fusion following conformational modifications in the endolysosomal compartment. 226 and 228 residues in hemagglutinin (HA) of the H3 subtype play a key role in host range restriction and receptor specificity. Influenza virus hemagglutinin (HA) is responsible for 1918 influenza pandemic.

Stoccaggio e stabilità

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable