T0440
3,3′,5,5′-Tetramethylbenzidine
peroxidase substrate, chromogenic, liquid
Sinonimo/i:
TMB
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About This Item
Codice UNSPSC:
12352204
NACRES:
NA.83
Prodotti consigliati
product name
Sistema a substrato liquido 3,3′,5,5′-Tetrametil-benzidina (TMB) per ELISA, peroxidase substrate
Livello qualitativo
Forma fisica
liquid
Temperatura di conservazione
2-8°C
Descrizione generale
TMB substrate is a chromogenic substrate for horseradish peroxidase - HRP.
Applicazioni
TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates. TMB substrate develops a soluble blue reaction product that can be read at 370 or 655 nm at 370 or 655 nm. The reaction is stopped with acid, forming a yellow reaction product which enables accurate intensity measurement at 450 nm.
How to use our TMB substrate:
How to use our TMB substrate:
- Our TMB substrate is supplied as a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. Prior to reaction with peroxidase, the substrate should be a colorless to light bluish-green solution. The substrate system develops a blue reaction product when reacted with peroxidase.
- Upon reaching your assay endpoint with addition of acid, measure the TMB substrate intensity at 450 nm.
- Our TMB Substrate is recommended for ELISA (microwell) procedures, and has been used in various assays, as a substrate for horseradish peroxidase antibody conjugate for detecting hendra virus glycoprotein, tetracosactide, sIgA and neutrophil extracellular traps (NETs).
- ′′ Since TMB substrate produces a soluble reaction product, it is not recommended for histochemistry or blotting.
Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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