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Key Documents

A0919

Sigma-Aldrich

Mouse IgG−Agarose

(Suspension in 0.5 M NaCl containing preservative.)

Sinonimo/i:

IgG-Agarose Beads

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Coniugato

agarose conjugate

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Forma fisica

(Suspension in 0.5 M NaCl containing preservative.)

Grado di funzionalizzazione

1 mg per mL

tecniche

immunoprecipitation (IP): suitable

Matrice

Cross-linked 4% beaded agarose

Attivazione matrice

cyanogen bromide

Braccio spaziatore

1 atom

Temperatura di conservazione

2-8°C

Descrizione generale

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

Applicazioni

IgG-Agarose can be used as an immunoadsorbent. It can also be used to affinity purify antibodies, remove species specific cross-reacting antibodies or remove contaminating antibodies from an antiserum preparation. Typically cross-reacting antibodies may be removed from an antiserum preparation using an equal volume of IgGAgarose (resin volume). Mouse IgG-Agarose has been used for several immunoprecipitation assays,.
Mouse IgGs conjugated to agarose beads were used in immunoprecipitation assays to pre-clear HEK 293 whole cell extracts. Beads were incubated with extracts for 1 hour rotating at 4°C.

Stato fisico

Suspension in 0.5 M NaCl containing preservative.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Atxn7, a subunit of SAGA chromatin remodeling complex, is subject to polyglutamine expansion at the amino terminus, causing spinocerebellar ataxia type 7 (SCA7), a progressive retinal and neurodegenerative disease. Within SAGA, the Atxn7 amino terminus anchors Non-stop, a deubiquitinase, to
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