P3296
Protein G Sepharose™, Fast Flow
recombinant, expressed in E. coli, aqueous ethanol suspension
Sinonimo/i:
Protein G-Agarose, Fast Flow from Streptococcus sp.
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About This Item
Prodotti consigliati
Ricombinante
expressed in E. coli
Livello qualitativo
Forma fisica
aqueous ethanol suspension
Classi chimiche degli analiti
proteins (Immunoglobulins of various mammalian species)
Grado di funzionalizzazione
~2 mg per mL
tecniche
affinity chromatography: suitable
Matrice
Sepharose 4B Fast Flow
Attivazione matrice
cyanogen bromide
Gruppi immobilizzati alla matrice
amino
Braccio spaziatore
1 atom
Temperatura di conservazione
2-8°C
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Descrizione generale
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.
P3296-5Ml′s updated product number is GE17-0618-01
P3296-5Ml′s updated product number is GE17-0618-01
Applicazioni
Protein G-Sepharose™ is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, protein A, G and L resins, protein interaction, and purification and detection. Protein G-Sepharose™ has been used to develop a strategy to confirm the presence of anti-erythropoietin neutralizing antibodies in human serum as well as to compare methods for depletion of albumin and IgG from equine serum.
Stato fisico
Suspension in 20% ethanol
Nota sulla preparazione
Prepared with recombinant streptococcal Protein G from which the albumin-binding region has been genetically deleted
Note legali
Sepharose is a trademark of Cytiva
Prodotti correlati
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Descrizione
Determinazione del prezzo
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Flam. Liq. 3
Codice della classe di stoccaggio
3 - Flammable liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
115.0 °F - closed cup
Punto d’infiammabilità (°C)
46.1 °C - closed cup
Certificati d'analisi (COA)
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Protocolli
To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.
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