GE17-0780-01
Protein A-Sepharose™ CL-4B
Cytiva 17-0780-01, pack of 1.5 g
Sinonimo/i:
Protein A Sepharose, Sepharose CL-4B
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About This Item
Codice UNSPSC:
41106500
NACRES:
NA.56
Prodotti consigliati
ligand
native protein A
Confezionamento
pack of 1.5 g
Produttore/marchio commerciale
Cytiva 17-0780-01
Condizioni di stoccaggio
(20% Ehtanol)
Matrice
4% cross-linked agarose
Dimensione particelle
45-165 μm
Diametro medio
90 μm
cleaning
2-10
Intervallo di esercizio
3-9
Capacità
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
Temperatura di conservazione
2-8°C
Categorie correlate
Descrizione generale
Protein A Sepharose™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Caratteristiche e vantaggi
- Well documented for immunoglobulin purification and fractionation
- Strong binding to Fc region of IgG
- High capacity - 20 mg human IgG/mL.
- Suited for immunoprecipitation procedures
Stoccaggio e stabilità
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Risultati analitici
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Note legali
Sepharose is a trademark of Cytiva
Codice della classe di stoccaggio
13 - Non Combustible Solids
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Certificati d'analisi (COA)
Lot/Batch Number
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