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P3391

Millipore

Protein A-Sepharose™ from Staphylococcus aureus

lyophilized powder

Sinonimo/i:

Protein A–Agarose

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About This Item

Numero MDL:
MFCD00165563
Codice UNSPSC:
41106500
NACRES:
NA.56

Forma fisica

lyophilized powder

Livello qualitativo

200

Confezionamento

glass bottle of 1 g
glass bottle of 1.5 g
glass bottle of 250 mg
glass bottle of 5 g

Condizioni di stoccaggio

do not freeze

Grado di funzionalizzazione

3 mg per mL

tecniche

affinity chromatography: suitable

Colore

white to gray

Matrice

Sepharose CL-4B

Attivazione matrice

cyanogen bromide

Gruppi immobilizzati alla matrice

amino

Braccio spaziatore

1 atom

Capacità

≥16 mg/mL binding capacity (human IgG)

Solubilità

water: 1 g/L at 20 °C

Compatibilità

conforms to structure for swelling capacity (1g swells to 4 to 5 mL)

Temperatura di conservazione

2-8°C

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Categorie correlate

Descrizione generale

Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.

Applicazioni

Protein A-Sepharose was used:
  • for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.
  • in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.
  • as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.
  • in immunoprecipitation of [32P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.
Protein A-Sepharose™ is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Quantità

Swelling: 1 g swells to approx. 4 ml.

Stato fisico

Supplied as lyophilized powder stabilized with lactose and dextran.

Note legali

Sepharose is a trademark of Cytiva

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Lionel Gissot et al.
Plant physiology, 142(3), 931-944 (2006-10-10)
The sucrose nonfermenting-1 protein kinase (SNF1)/AMP-activated protein kinase subfamily plays a central role in metabolic responses to nutritional and environmental stresses. In yeast (Saccharomyces cerevisiae) and mammals, the beta- and gamma-noncatalytic subunits are implicated in substrate specificity and subcellular localization
P Hornbeck et al.
Molecular and cellular biology, 9(9), 3727-3735 (1989-09-01)
The regulation and expression of protein kinase C (PKC) and phosphomyristin C (PMC) (a principal substrate of PKC which is the major myristylated protein in lymphocyte and glioma lines that express it) in murine B and T lymphocytes were investigated.
M Iezzi et al.
Molecular endocrinology (Baltimore, Md.), 13(2), 202-212 (1999-02-11)
Insulin-secreting cells express four GTPases of the Rab3 family. After separation of extracts of INS-1 cells on a sucrose density gradient, the bulk of the A, B, and C isoforms was recovered in the fractions enriched in insulin-containing secretory granules.
Adam F L Hurlstone et al.
The Biochemical journal, 364(Pt 1), 255-264 (2002-05-04)
A highly conserved multisubunit enzymic complex, SWI/SNF, participates in the regulation of eukaryote gene expression through its ability to remodel chromatin. While a single component of SWI/SNF, Swi2 or a related protein, can perform this function in vitro, the other
Peter Oliviusson et al.
The Plant cell, 18(5), 1239-1252 (2006-04-04)
Receptors for acid hydrolases destined for the lytic compartment in yeast and mammalian cells are retrieved from intermediate, endosomal organelles with the help of a pentameric protein complex called the retromer. We cloned the Arabidopsis thaliana homologs of the three
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Protocolli

Immunoprecipitation Procedure

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

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