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Key Documents

14326C

SAFC

EX-CELL® 302 Serum-Free Medium for CHO Cells

without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Produzione farmaceutica

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About This Item

Numero MDL:
Codice UNSPSC:
12352207
NACRES:
NA.75

Descrizione

for research or for further manufacturing use

Livello qualitativo

Sterilità

sterile-filtered

Nome Commerciale

EX-CELL®

Forma fisica

liquid

tecniche

cell culture | mammalian: suitable (suspension)

Componenti

HEPES: yes
phenol red: no
L-glutamine: no
NaHCO3: yes
glucose: yes

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Descrizione generale

EX-CELL® 302 is a serum-free liquid medium, which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL® 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or the GS System, because it does not contain hypoxanthine, thymidine or L-glutamine. If EX-CELL® 302 is to be used with CHO cells transformed using the GS selection the media needs to be supplemented with GS Supplement 50X (Catalog No. 58672C) for the additional amino acids and nucleosides. For use with DHFR- selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL® 302 was developed using only recombinant human proteins that have molecular weights less than 10 kD. The total protein concentration found in EX-CELL® 302 is less than 1 mg/L. Pluronic® F68 has been added at a final concentration of 0.1% to EX-CELL® 302 to protect against shear damage in sparged bioreactor systems. Catalog No. 14326C replaces Catalog No. 14313 and includes an alternate source of soy hydrolysate to that found in the original EX-CELL® 302 formulation. With more consistent performance and improved filtration characteristics, the alternate hydrolysate will improve the overall performance and consistency of EX-CELL® 302. Comparability testing utilizing the previous soy hydrolysate and the replacement hydrolysate demonstrated comparable growth promoting characteristics.

Avvertenza

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.

Note legali

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
GS System is a trademark of Lonza Group Ltd.
Pluronic is a registered trademark of BASF

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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