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P4864

Sigma-Aldrich

Propidium iodide solution

≥94% purity, solution (1.0 mg/ml in water)

Sinonimo/i:

3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide, 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium, diiodide

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About This Item

Formula empirica (notazione di Hill):
C27H34I2N4
Numero CAS:
25535-16-4
Peso molecolare:
668.39
Beilstein:
3843838
Numero MDL:
MFCD00011921
Codice UNSPSC:
12171500
ID PubChem:
24898563
NACRES:
NA.47

Nome del prodotto

Propidium iodide solution,

Saggio

≥94%

Livello qualitativo

200

Stato

liquid

tecniche

titration: suitable

Colore

faint orange to very dark orange, and Faint Red to Very Dark Red and Faint Purple to Very Dark Purple and Orange-Red and Red-Orange

Solubilità

water: 1 mg/mL

εmax

5400-6400 at 491-495 nm in phosphate buffer

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

2-8°C

Stringa SMILE

[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
XJMOSONTPMZWPB-UHFFFAOYSA-M

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Descrizione generale

Propidium iodide solution is used to assess neuronal death.

Applicazioni

Membrane impermeant, phenanthridinium intercalator dye excited by mercury or xenon-arc lamps or argon-ion laser. Suitable for use in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry. Used as a nuclear counterstain.
Propidium iodide solution has been used:
  • in staining DNA
  • in the viability staining method to study islet viability
  • to visualize F-actins and nuclei in mesenchymal stem cell sheets

Altre note

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves

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Certificati d'analisi (COA)

Lot/Batch Number
MKCM8441
MKCK5162
MKCC9922
MKBV9923V
SLBB4626V

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Iva Slaninová et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 71(9), 700-708 (2007-06-06)
Quaternary benzo[c]phenanthridine alkaloids (QBAs) are naturally occurring compounds isolated from plants in the Fumariaceae, Papaveraceae, Ranunculaceae, and Rutaceae families. In addition to having a wide range of biological activities, they are also attractive for their fluorescent properties. We observed interesting
Stroke E-Book: Pathophysiology Diagnosis and Management, 79-79 (2011)
Variation in human islet viability based on different membrane integrity stains
Barnett MJ, et al.
Cell Transplantation, 13(5), 481-488 (2004)
Yonggang Yang et al.
Scientific reports, 5, 18583-18583 (2015-12-22)
Viability is a common issue of concern in almost all microbial processes. Fluorescence-based assays are extensively used in microbial viability assessment, especially for mixed-species samples or biofilms. Propidium iodide (PI) is the most frequently used fluorescence indicator for cell viability
Chung-Chi Wang et al.
Cardiovascular research, 77(3), 515-524 (2007-11-17)
Cell transplantation is a promising approach for patients with myocardial infarction. However, following injection, retention of the transplanted cells in the injected area remains a central issue, which can be deleterious to cell transplantation therapy. We hypothesized that the use
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