537059
Propidium Iodide
Membrane impermeable DNA intercalator.
Sinonimo/i:
Propidium Iodide
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Formula empirica (notazione di Hill):
C27H34I2N4
Numero CAS:
Peso molecolare:
668.39
Numero MDL:
Codice UNSPSC:
12352200
NACRES:
NA.84
Prodotti consigliati
Livello qualitativo
Forma fisica
solid
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
protect from light
Colore
dark red
input
sample type intact cells
Fluorescenza
λex ~536 nm
λem ~617 nm
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
XJMOSONTPMZWPB-UHFFFAOYSA-M
Descrizione generale
Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M-1cm-1 (493 nm); 55,700 M-1cm-1 (287 nm); 13,800 M-1cm-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.
Azioni biochim/fisiol
Cell permeable: no
Primary Target
Membrane impermeable DNA intercalator
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no
Attenzione
Toxicity: Irritant (B)
Nota sulla preparazione
Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.
Ricostituzione
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Altre note
Belloc, F., et al. 1994. Cytometry 17, 59.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Muta. 2
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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