60983

Kovac′s reagent for indoles

suitable for microbiology

• Sinonimo/i: 4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac
• Formula empirica (notazione di Hill): C₉H₁₁NO
• Numero CAS: 100-10-7
• Peso molecolare: 149.19
• Beilstein: 4132845
• Numero MDL: MFCD00003381
• Codice UNSPSC: 41171621
• ID PubChem: 329759335
• NACRES: NA.85

Proprietà

• Durata: limited shelf life, expiry date on the label
• Livello qualitativo: 100
• Composizione: n-butanol ; 4-dimethylaminobenzaldehyde ; hydrochloric acid
• tecniche: microbe id | specific enzyme detection: suitable
• Indice di rifrazione: n20/D 1.400-1.420
• applicazioni: agriculture; clinical testing; environmental; food and beverages; microbiology
• Temperatura di conservazione: 2-8°C
• Compatibilità: Escherichia coli; coliforms
• Stringa SMILE: [H]C(=O)c1ccc(cc1)N(C)C
• InChI: 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3; BGNGWHSBYQYVRX-UHFFFAOYSA-N

Descrizione

Descrizione generale

Kovac′s reagent for indoles is a solution of 4-(dimethylamino)benzaldehyde and hydrochloric acid in n-butanol.

Applicazioni

Kovac′s reagent for indoles has been used for enumerating E. coli O157:H7 in ground beef samples in different scenarios of stored, thawed, and as cooked patty samples. It has also been used for E coli enumeration in dewatered blackwater.

Azioni biochim/fisiol

Kovac′s reagent detects the indole and aids in identifying the indole-positive and indole-negative microorganisms. E. coli can split tryptophan into indole and α-aminopropionic acid in the presence of the enzyme tryptophanase. p-Aminobenzaldehyde present in the reagent reacts with indole to form a cherry-red complex, that is soluble in alcohol, ether, and chloroform. It is recommended to use glucose free media and peptone with a high tryptophan content for the test.

Informazioni sulla sicurezza

• Pittogrammi: GHS02,GHS05,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H226,H290,H302,H315,H318,H335,H336
• Consigli di prudenza: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Central nervous system, Respiratory system
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): 96.8 °F
• Punto d’infiammabilità (°C): 36 °C
• Dispositivi di protezione individuale: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter