1.11350

Bactident^™ Kovac′s Indole reagent

Available in a practical dropper bottle, for Escherichia coli

• Sinonimo/i: 4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent
• Codice UNSPSC: 41106200
• NACRES: NA.84

Proprietà

• Livello qualitativo: 100
• Descrizione: Bactident™ E. coli 1.13303
• Forma fisica: liquid (ready-to-use)
• Durata: 210 days
• Caratteristiche: ready-to-use
• Composizione: (n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)
• Confezionamento: pkg of (1 x 30 ml, Plastic bottle)
• tecniche: microbe id | specific enzyme detection: suitable
• pH: <1 (20 °C in H₂O)
• Temp. transizione: flash point 36 °C
• Densità: 0.92 g/cm³ at 20 °C
• applicazioni: environmental; food and beverages
• Temperatura di conservazione: 2-8°C
• Compatibilità: Enterobacter spp.; Escherichia coli

Descrizione

Descrizione generale

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

Applicazioni

Bactident^™ Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

Linkage

Replaces: 1.11350.0007

Risultati analitici

Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.

Note legali

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS02,GHS05,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H226 - H290 - H302 - H314 - H317 - H335 - H336
• Consigli di prudenza: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): 96.8 °F
• Punto d’infiammabilità (°C): 36 °C