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Merck
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Documenti

11034731001

Roche

Primer "random"

lyophilized, sufficient for ≤400 reactions, pkg of 2 mg (Primer, Random pd(N)6 Potassium Salt)

Sinonimo/i:

primer

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
41106300

Forma fisica

lyophilized

Livello qualitativo

impiego

sufficient for ≤400 reactions

Confezionamento

pkg of 2 mg (Primer, Random pd(N)6 Potassium Salt)

Produttore/marchio commerciale

Roche

Temperatura di conservazione

−20°C

Descrizione generale

p(dN)6

Applicazioni

Primer "random" has been used:
  • in a reverse transcription-polymerase chain reaction (RT-PCR) and quantitative PCR (qPCR) to retro-transcribe RNA
  • to reverse-transcribe RNA samples to cDNA
  • in digital droplet PCR (ddPCR) to synthesis cDNA from RNA

Primer for cDNA synthesis

Caratteristiche e vantaggi

  • Offers a consistent representation of all RNA sequences in mRNA
  • Can prime cDNA transcription from RNAs lacking a poly(A) tail
  • Short cDNA transcripts can overcome the challenges posed by RNA secondary structures
  • The average length of cDNAs formed can be controlled by adjusting the ratio of random primers to RNA in the reverse transcription (RT) reaction

Altre note

For life science research only. Not for use in diagnostic procedures.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash


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Amaresh C Panda et al.
Nucleic acids research, 44(5), 2393-2408 (2016-01-29)
Skeletal muscle contains long multinucleated and contractile structures known as muscle fibers, which arise from the fusion of myoblasts into multinucleated myotubes during myogenesis. The myogenic regulatory factor (MRF) MYF5 is the earliest to be expressed during myogenesis and functions
Monika Hiller et al.
PloS one, 13(10), e0204485-e0204485 (2018-10-03)
Duchenne muscular dystrophy is a lethal disease caused by lack of dystrophin. Skipping of exons adjacent to out-of-frame deletions has proven to restore dystrophin expression in Duchenne patients. Exon 51 has been the most studied target in both preclinical and
Vadim Alexandrov et al.
Nature biotechnology, 34(8), 838-844 (2016-07-05)
Rapid technological advances for the frequent monitoring of health parameters have raised the intriguing possibility that an individual's genotype could be predicted from phenotypic data alone. Here we used a machine learning approach to analyze the phenotypic effects of polymorphic
Tae-Dong Kim et al.
The Journal of clinical investigation, 126(2), 706-720 (2016-01-06)
Histone demethylase upregulation has been observed in human cancers, yet it is unknown whether this is a bystander event or a driver of tumorigenesis. We found that overexpression of lysine-specific demethylase 4A (KDM4A, also known as JMJD2A) was positively correlated
Marie Rieux et al.
Molecular psychiatry, 26(9), 5441-5463 (2020-06-10)
Huntington's disease (HD) is a monogenic neurodegenerative disorder resulting from a mutation in the huntingtin gene. This leads to the expression of the mutant huntingtin protein (mHTT) which provokes pathological changes in both the central nervous system (CNS) and periphery.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.