MABN2295

Anti-Beta (β)-Amyloid antibody

rat monoclonal, 2E9

• Sinonimo/i: ABPP, APPI, APP, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Beta-amyloid precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Proprietà

• Nome del prodotto: Anti-Amyloid beta A4 protein Antibody, clone 2E9, clone 2E9, from rat
• Origine biologica: rat
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 2E9, monoclonal
• Reattività contro le specie: mouse, human
• tecniche: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_000475
• N° accesso UniProt: P05067
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... APP(351)

Descrizione

Descrizione generale

Amyloid beta A4 protein (UniProt: P05067; also known as ABPP, APPI. APP, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Beta-amyloid precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. Deposition of Amyloid beta (Ab) is an early event in the pathogenesis of Alzheimer s disease (AD). Ab peptides originate from the proteolytic cleavage of the amyloid precursor protein (APP). APPs are cell surface protein that are internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha-secretase cleavage, soluble APP is released into the extracellular space and the C-terminal is internalized to endosomes and lysosomes. The beta-secretase cleaves APP between residues Met671 and Asp672 and yields sAPP beta and C99. Following the beta-secretase cleavage, a second cleavage occurs at the C-terminus of Ab peptide that releases Ab from C99. This cleavage occurs in the vicinity of residue 712 of the C-terminus. The gamma-secretase can cleave the C-terminal region at either Val711 or Ile713 to produce the shorter Ab peptide (Ab1-40) or the longer Ab peptide (Ab1-42). Ab1-42 occurs more frequently and forms fibrillar aggregates far more readily than the Ab1-40 peptide. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu2+ and Fe3+ to Cu+ and Fe2+, respectively. Abeta42 is a more effective reductant than beta-amyloid 40. From a physiological point of view, it functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. It is also involved in cell mobility and transcription regulation through protein-protein interactions and can promote transcription activation through binding to APBB1-KAT5.

Specificità

Clone 2E9 detects multiple isoforms of APP. It targets a sequence of 11 amino acids in the C-terminal region.

Immunogeno

KLH-conjugated linear peptide corresponding to 11 amino acids from the C-terminal half of human Amyloid beta A4 protein (APP770).

Applicazioni

Anti-Amyloid beta A4 protein, clone 2E9 Antibody, Cat. No. MABN2295, is a highly specific rat monoclonal antibody that targets Amyloid beta A4 protein and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Amyloid beta A4 protein in 10 µg of N2a, N2aA, and N2aAB cell lysate.

Immunofluorescence Analysis: A representative lot detected Amyloid beta A4 protein in Immunofluorescence applications (Willem, M., et. al. (2015). Nature. 526(7573):443-7).

Western Blotting Analysis: A representative lot detected Amyloid beta A4 protein in Western Blotting applications (Willem, M., et. al. (2015). Nature. 526(7573):443-7).

Immunoprecipitation Analysis: A representative lot detected Amyloid beta A4 protein in Immunoprecipitation applications (Willem, M., et. al. (2015). Nature. 526(7573):443-7).

Qualità

Evaluated by Western Blotting in human Ntera-2 cell lysate.

Western Blotting Analysis: 0.5 ug/mL of this antibody detected Amyloid beta A4 protein in 10 µg of human Ntera-2 cell lysate

Descrizione del bersaglio

~110 kDa observed. Calculated 86.94 kDa for APP770, 78.66 kDa for APP695. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable