437996

5-Lipoxygenase, Human, Recombinant, S. fruigiperda

5-Lipoxygenase, Human, Recombinant, S. frugiperda E.C 1.13.11.34, catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4.

• Sinonimo/i: 5-LO
• Classificazione EC (Enzyme Commission): 1.13.11.34 (BRENDA, IUBMB)
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Livello qualitativo: 100
• Forma fisica: liquid
• Attività specifica: ~20 U/mg
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

Recombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. 5-LO catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A₄. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA₂.

Recombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. It is localized in the cytosol of resting neutrophils, and is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA₂.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25°C in 50 mM Tris-HCl, 100 µM arachidonate, 2 mM CaCl₂, 1 mM ATP, 250 µM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode.

Stato fisico

In 100 mM Tris containing 5 mM EGTA, pH 8.0.

Ricostituzione

Following initial thaw, aliquot and freeze (-70°C).

Altre note

Brock, T.G., et al. 1997. J. Biol. Chem.272, 8276.
Pouliot, M., et al. 1996. Eur. J. Biochem.238, 250.
Zhang, Y.Y., et al. 1993. J. Biol. Chem.268, 2535.
Shimizu, T., et al. 1984. Proc. Natl. Acad. Sci. USA81, 689.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable