C6423

αα-Chymotrypsin aus Rinderpankreas

suitable for protein sequencing, salt-free, lyophilized powder

• CAS-Nummer: 9004-07-3
• EC-Nummer: 3.4.21.1 (BRENDA, IUBMB)
• EG-Nummer: 232-671-2
• MDL-Nummer: MFCD00130481
• UNSPSC-Code: 12352204
• eCl@ss: 42010112
• NACRES: NA.26

Eigenschaften

• Qualität: Proteomics Grade
• Qualitätsniveau: 200
• Form: salt-free, lyophilized powder
• Mol-Gew.: 25 kDa
• Eignung: suitable for protein sequencing
• UniProt-Hinterlegungsnummer: P00767
• Lagertemp.: 2-8°C
• Angaben zum Gen: cow ... CTRB1(618826)

Beschreibung

Allgemeine Beschreibung

α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein. The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.

Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry. It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.

Anwendung

α-Chymotrypsin from bovine pancreas is used for the following applications:

• Protein Identification by mass spectrometry (MS)
• The isolation and characterization of myosin heavy chains
• Toxin preparation
• The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE

α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction.

Biochem./physiol. Wirkung

α-Chymotrypsin from bovine pancreas is stabilized by Ca²⁺ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side. α-Chymotrypsin is inhibited by Cu²⁺ and Hg.

Ein Serin-Protease, die Peptidbindungen mit aromatischen oder großen hydrophoben Seitenketten (Tyr, Trp, Phe, Met, Leu) am Carboxylende der Bindung hydrolysiert.

Einheitendefinition

Eine Einheit hydrolysiert 1.0 μmol BTEE pro min bei pH 7.8 und 25 °C.

Sonstige Hinweise

Finden Sie mehr Informationen zu Chymotrypsin auf unserer 'Enzyme-Explorer'-Webseite.

Sicherheitshinweise

• Piktogramme: GHS07,GHS08,GHS09
• Signalwort: Danger
• H-Sätze: H302 - H315 - H319 - H334 - H335 - H400
• P-Sätze: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Faceshields, Gloves