T9777

Trypsin-chymotrypsin inhibitor

lyophilized powder

• Synonym(e): Bowman-Birk Inhibitor
• CAS-Nummer: 37330-34-0
• MDL-Nummer: MFCD00132532
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• product name: Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder
• Biologische Quelle: Glycine max (soybean)
• Qualitätsniveau: 200
• Form: lyophilized powder
• Zusammensetzung: Protein, 70-90% biuret
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Trypsin-chymotrypsin inhibitor belongs to the family of plant protease inhibitors. It is mainly found in the legume (Fabaceae) and cereal (Poaceae) families. Trypsin-chymotrypsin inhibitor consists of many internal disulfide bonds between conserved Cys residues, which maintains structural stability and active conformation of the inhibitory loops. Trypsin-chymotrypsin has anticarcinogenic properties.

Highly cross-linked with seven disulfide bridges, Bowman-Birk inhibitor has spatially distinct domains for trypsin and chymotrypsin inhibition.

Anwendung

Bowman Birk protease inhibitor prevents radiation-induced carcinogenesis by a reduction of incorrect DNA repairs, resulting in a reduced amount of dicentric chromosomes.

Trypsin-chymotrypsin inhibitor from Glycine max (soybean) has been used for flow cytometric separase activity assay. It has also been used to inhibit trypsin and chymotrypsin for in vitro gastrointestinal (GI) digestion studies.

Einheitendefinition

One trypsin unit = ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C. Reaction volume = 3.2 mL (1 cm light path).

Physikalische Form

Lyophilized powder containing phosphate buffer salts, pH 7.6

Hinweis zur Analyse

1 mg protein will inhibit ≥0.5 mg trypsin with activity of ~10,000 BAEE units per mg protein or ≥1.0 mg chymotrypsin with activity of ~40 BTEE units per mg protein.

Sonstige Hinweise

View more information on Trypsin Inhibitor.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)