10109886001

Trypsin Inhibitor

from soybean

• Synonym(e): trypsin
• UNSPSC-Code: 12352207

Eigenschaften

• Biologische Quelle: soybean
• Qualitätsniveau: 100
• Form: lyophilized
• Mol-Gew.: M_r 20,100
• Verpackung: pkg of 50 mg
• Hersteller/Markenname: Roche
• Konzentration: 1 mg/mL
• Methode(n): cell culture | mammalian: suitable
• pH-Wert: 3.8-4.5
• Löslichkeit: water: soluble
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.

Spezifität

Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).

Anwendung

Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.

Trypsin Inhibitor has been used:

• in the buffer for hepatocyte isolation
• to inhibit trypsin in Corneal epithelial cells
• to inhibit papain activity while treating hippocampal neural stem cells (NSCs)

Biochem./physiol. Wirkung

Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.

Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.

Spezifikationen

Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)

Isoelectric point: 4.5

Einheitendefinition

1 U = 270 S & T-units or 90 USP-units

Angaben zur Herstellung

Working concentration: 10 to 100 μg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.

Rekonstituierung

Sensitive to heat, alkaline pH, and protein-precipitating compounds.

Lagerung und Haltbarkeit

Store at 2 to 8 °C. (Store dry!)

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable