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A DNA-based molecular probe for optically reporting cellular traction forces.

A DNA-based molecular probe for optically reporting cellular traction forces.

Nature methods (2014-10-13)

Brandon L Blakely, Christoph E Dumelin, Britta Trappmann, Lynn M McGregor, Colin K Choi, Peter C Anthony, Van K Duesterberg, Brendon M Baker, Steven M Block, David R Liu, Christopher S Chen

RESUMO

We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges.

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Acetonitrilo, suitable for HPLC, gradient grade, ≥99.9%

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Dimetilsulfóxido, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

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Ácido trifluoracético, ReagentPlus^®, 99%

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Dimetilsulfóxido, ReagentPlus^®, ≥99.5%

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Metanol, HPLC Plus, ≥99.9%

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Ácido trifluoracético, puriss. p.a., suitable for HPLC, ≥99.0% (GC)

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Acetonitrilo, ≥99.9% (GC)

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Dimetilsulfóxido, puriss. p.a., ACS reagent, ≥99.9% (GC)

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