Y2146

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, and tryptophan

• Sinônimo(s): Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements, Yeast media, amino acid dropout supplements
• Número MDL: MFCD01095679
• Código UNSPSC: 12352200
• NACRES: NA.85

Propriedades

• Nível de qualidade: 200
• forma: powder
• técnica(s): transformation: suitable
• aplicação(ões): microbiology
• temperatura de armazenamento: room temp

Descrição

Aplicação

Yeast Synthetic Drop-out Medium Supplements are suitable for screening yeast transformants.

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Outras notas

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Nota de preparo

Dissolve 1.46 g of supplement per liter of medium.

Informações de segurança

• Pictogramas: GHS05
• Palavra indicadora: Danger
• Frases de perigo: H318
• Declarações de precaução: P280 - P305 + P351 + P338
• Classificações de perigo: Eye Dam. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable