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Key Documents

Y1500

Sigma-Aldrich

YPD Agar

Sinônimo(s):

Yeast Extract Peptone Dextrose Agar, YEPD Agar

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About This Item

Código UNSPSC:
50405901
eCl@ss:
42040102
NACRES:
NA.75

forma

powder

Nível de qualidade

composição

Agar, 15 g/L
Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L

aplicação(ões)

food and beverages

adequação

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

Descrição geral

YPD agar is a complete yeast culture medium for most yeast species, especially for Saccharomyces cerevisiae. It is also known as YEPD and comprises of yeast extract, bacteriological peptone, glucose, and agar. The components of this media provide all the necessary nutrients for yeast growth. The essential vitamins, minerals and amino acids in the medium acts carbon and nitrogen source.

Aplicação

YPD agar has been used as a culture media:
  • to cultivate S. cerevisiae strains to find a long-term preservation method for a deep space biosensor mission
  • for yeast strains in in vitro broth microdilution (BMD) based on minimum inhibitory concentration (MIC)
  • for Zymoseptori triciti strains for virulence studies

Nota de preparo

1. Suspend 65 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Artigos

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

Protocolos

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

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