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Documentos Principais

17222

Millipore

WL Nutrient Agar

suitable for microbiology, NutriSelect® Plus,

for cultivation of microorganisms encountered in the brewing and fermentation industries

Sinônimo(s):

Wallerstein Laboratory Nutrient Agar

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About This Item

Código UNSPSC:
41171605
NACRES:
NA.74

Nível de qualidade

esterilidade

non-sterile

Formulário

powder

prazo de validade

limited shelf life, expiry date on the label

composição

agar, 20 g/L
bromocresol green, 0.022 g/L
calcium chloride, 0.125 g/L
casein enzymic hydrolysate, 5 g/L
dextrose, 50 g/L
ferric chloride, 0.0025 g/L
magnesium sulfate, 0.125 g/L
manganese sulfate, 0.0025 g/L
monopotassium phosphate, 0.55 g/L
potassium chloride, 0.425 g/L
yeast extract, 4 g/L

fabricante/nome comercial

NutriSelect® Plus

técnica(s)

microbiological culture: suitable

pH final

5.5±0.2 (25 °C)

aplicação(ões)

bioburden testing
food and beverages

microbiology

adequação

nonselective and differential for Candida spp.
nonselective and differential for Escherichia coli
nonselective and differential for Lactobacillus spp.
nonselective and differential for Pichia spp.
nonselective and differential for Proteus spp.
nonselective and differential for Saccharomyces spp.
nonselective and differential for Zygosaccharomyces spp.
nonselective and differential for bacteria (General Media)
nonselective and differential for coliforms
nonselective and differential for molds (General Media)
nonselective and differential for yeasts (General Media)

Descrição geral

WL (Walerstein Laboratory) media follow the formula described by Green and Gray for the examination of materials related to brewing and for industrial fermentations containing mixed flora of yeasts and bacteria. Yeast extract is a source of vitamins, amino acids and trace elements. Casein enzymic hydrolysate provides nitrogen, amino acids and carbon. Dextrose is the main carbon and energy source. Potassium phosphate is the buffering agent. Several salts in the medium provide essential ions and help to maintain osmotic balance. Bromcresol green acts as pH indicator and agar as solidifying agent. WL Nutrient Agar is used for the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeasts and bacteria.

Aplicação

WL Nutrient Agar is suitable:
  • for reviving yeast stock cultures and for population dynamics studies post fermentation
  • for morphological characterization of yeast isolated from cheese
  • for plating fermented wine for yeast species isolation

Nota de preparo

Suspend 80.25 g in 1 litre distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. If desired, to obtain a pH of 6.5, add 1% solution of sodium bicarbonate.

Nota de rodapé

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informações legais

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Paper read at American Society of Brewing Chemists Meeting
Green, SR and Gray, PP
Wallerstein Laboratories Communications, 12, 43-43 (1950)
The impact of Saccharomyces cerevisiae on a wine yeast consortium in natural and inoculated fermentations
Bagheri B, et al.
Frontiers in Microbiology, 8, 1988-1988 (2017)
The rapid detection of brewery spoilage micro-organisms
Harrison J, et al.
Journal of the Institute of Brewing, 80(4), 390-398 (1974)
Selection of autochthonous Saccharomyces cerevisiae strains for production of typical Pinot Gris wines.
Schvarczova E, et al.
Journal of Food & Nutrition Research, 56(4) (2017)
Characterisation of yeast flora isolated from an artisanal Portuguese ewe's cheese
Malfeito-Ferreira M, et al.
International Journal of Food Microbiology (2000)

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