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Sigma-Aldrich

Extract-N-Amp Tissue PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

Sinônimo(s):

Tissue direct PCR kit

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About This Item

Código UNSPSC:
41106303
NACRES:
NA.55

uso

sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions

Nível de qualidade

Características

dNTPs included
hotstart

técnica(s)

PCR: suitable

cor

colorless

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Procurando produtos similares? Visita Guia de comparação de produtos

Descrição geral

The Extract-N-Amp Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by direct PCR (polymerase chain reaction) . A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR ReadyMix for amplification directly from the extract.

Aplicação

Extract-N-Amp tissue PCR kit has been used for genomic DNA extraction from mouse tail biopsies and polymerase chain reaction (PCR).

Características e benefícios

  • Fast – rapid extraction of genomic DNA for PCR in 15 minutes
  • Convenient – no long enzymatic digestions or column purification required
  • Practical – perfect for quick genomic DNA isolation for genotyping
  • Flexible – protocols available for mouse-tails, hair, animal tissue, saliva, and buccal swabs
  • Specific – Hot start antibody for highly specific PCR amplification of genomic DNA
  • Extract stable at 4 oC for at least 6 months

Componentes

Kits contain Tissue Preparation Solution, Extraction Solution, and Extract-N-Amp PCR Reaction Mix.

Princípio

The kit contains validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and Extract-N-Amp PCR ReadyMix, included in the kit.

Outras notas

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Informações legais

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Palavra indicadora

Danger

Classificações de perigo

Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Protocolos

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

Conteúdo relacionado

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

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