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Key Documents

N3910

Sigma-Aldrich

Neutralization Solution B

Sinônimo(s):

DNA neutralization solution, neutralization buffer DNA extraction

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About This Item

Código UNSPSC:
12161703
NACRES:
NA.25

grau

for molecular biology

Nível de qualidade

forma

liquid

técnica(s)

DNA amplification: suitable
DNA extraction: suitable

temperatura de armazenamento

−20°C

Descrição geral

This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.

Aplicação

Neutralization Solution B has been used in the modified Sigma Extract-N-Amp extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp PCR Reaction Mix.

Informações legais

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Pictogramas

Exclamation markEnvironment

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Protocolos

The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

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