X100RS

Triton^™ X-100 reduced

• Sinônimo(s): Polyoxyethylene (10) isooctylcyclohexyl ether
• Fórmula linear: 4-(C₈H₁₇)C₆H₁₀(OCH₂CH₂)_nOH, n~10
• Número CAS: 92046-34-9
• Número MDL: MFCD00167048
• Código UNSPSC: 12161900
• NACRES: NA.25

Propriedades

• fonte biológica: synthetic (oragnic)
• Nível de qualidade: 100
• descrição: non-ionic
• forma: viscous liquid
• peso molecular: micellar average mol wt 80,000; average mol wt 625
• número de agregação: 100-155
• índice de refração: n20/D 1.473 (lit.)
• CMC: 0.2-0.9
• pf: 48 -50 °C (118 - 122 °F)
• temperatura de transição: cloud point 65 °C
• solubilidade: water: soluble, clear to slightly hazy, colorless
• densidade: 1.029 g/mL at 25 °C (lit.)
• absorção: ≤0.25 at 277 nm in H₂O at 0.5%
• HLB: 13.5
• cadeia de caracteres SMILES: CC(C)(C)CC(C)(C)C1CCC(CC1)OCCOCCOCCOCCOCCOCCOCCO
• InChI: 1S/C28H56O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h25-26,29H,6-24H2,1-5H3
• chave InChI: QQJNBKDKLMCALZ-UHFFFAOYSA-N

Descrição

Descrição geral

Triton^™ X-100 reduced is a versatile nonionic surfactant featuring a hydrophilic polyethylene oxide chain, commonly employed as a laboratory detergent. Its applications span various purposes, including cell lysis to extract proteins or organelles, permeabilizing the membranes of living cells, and serving as a vital component in lysis buffers. Widely utilized in biochemical and cell biology research, Triton^™ X-100 facilitates the isolation of lipid rafts, enhancing the solubility and dispersibility of substances. With a reduced polyoxyethylene content of approximately 10, this product exhibits excellent wetting properties and emulsification enhancement.

In cell biology, Triton^™ X-100 plays a crucial role in solubilizing membrane-bound proteins, preserving their native conformation in solution. Furthermore, Triton^™ X-100 reduced, resulting from the full hydrogenation of the benzene moiety of TX-100 to a cyclohexane derivative, has been associated with potential benefits in enzyme digestion enhancement and effects on the photoisomerization of bacteriorhodopsin. The versatility of Triton^™ X-100 in various research applications underscores its significance as a fundamental tool in biochemical and cell biology investigations.

Aplicação

Triton^™ X-100 reduced has been used:

• in nuclear magnetic resonance (NMR) sample tube for lysing/rupturing the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) phospholipid vesicles
• in cell permeabilization
• as a component in Tris/HCl to determine hydrolysis of the esters for enzyme activity assays

Características e benefícios

• Non-ionic surfactant
• Improves solubility and dispersibility of substances
• Excellent wetting properties
• Enhances emulsification
• High purity product for research applications

Nota de preparo

Hydrogenated to reduce UV absorbance.

Outras notas

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Informações legais

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Informações de segurança

• Pictogramas: GHS07,GHS09
• Palavra indicadora: Warning
• Frases de perigo: H315,H319,H335,H411
• Declarações de precaução: P261 - P264 - P271 - P273 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): 235.4 °F - closed cup
• Ponto de fulgor (°C): 113 °C - closed cup
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves