SRP6273

UPA human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Sinônimo(s): ATF, BDPLT5, PLAU, QPD, UPA, URK, Urokinase, u-PA
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in HEK 293 cells
• etiqueta: 6-His tagged (C-terminus)
• Ensaio: ≥95% (SDS-PAGE)
• forma: lyophilized powder
• peso molecular: calculated mol wt 45 kDa; observed mol wt 18 kDa; observed mol wt 32 kDa; observed mol wt 50 kDa
• embalagem: pkg of 10 μg; pkg of 50 μg
• Impurezas: <1 EU/μg endotoxin (LAL test)
• nº de adesão UniProt: P00749
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... uPA(5328)

Descrição

Descrição geral

DTT-reduced. Protein migrates as three bands corresponding to the long α chain, β chain and unprocessed full-length chain due to glycosylation and cleavage. Ser21, Ile179 & Lys156 is the predicted N-terminus.

The PLAU (plasminogen activator, urokinase) gene is mapped to human chromosome 10q22.2.

Urokinase-type plasminogen activator is also known as PLAU and uPA. The human uPA is initially synthesized as 431 amino acid precursor with an N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule of different molecular weights. Two forms of the A chain exist, starting at Ser21 (the long form) and Lys156 (the short form). The long and short A chains are unique to the high and low molecular weight forms, respectively. The long A chain contains an EGF (epidermal growth factor)-like domain, responsible for binding of the PLAU receptor. The B chain corresponds to the catalytic domain. The gene encoding it is localized on human chromosome 10.

Ações bioquímicas/fisiológicas

Urokinase-type plasminogen activator is a serine protease with extremely limited substrate specificity, cleaving the sequence Cys-Pro-Gly-Arg560-Val561-Val-Gly-Gly-Cys in plasminogen to form plasmin. It is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. uPA (plasminogen activator, urokinase) - mediated degradation of extracellular matrix of migrating cells is essential for tissue remodeling, invasiveness and angiogenesis. uPA promotes pathogenesis of Psoriasis and basal cell carcinoma. uPA activates growth factors and metalloproteinases.

forma física

Lyophilized from 0.22 μm filtered solution in HEPES, NaCl and CaCl2. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable