Pular para o conteúdo
Merck
Todas as fotos(1)

Key Documents

SRP3312

Sigma-Aldrich

IL-10 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Sinônimo(s):

B-TCGF, CSIF, TGIF

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

fonte biológica

human

recombinante

expressed in E. coli

Ensaio

≥98% (HPLC)
≥98% (SDS-PAGE)

forma

lyophilized

potência

≤2.0 ng/mL

peso molecular

18.6 kDa

embalagem

pkg of 10 μg

Impurezas

endotoxin, tested

nº de adesão UniProt

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... IL10(3586)

Descrição geral

Interleukin-10 (IL-10) is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. The gene encoding this protein is localized on human chromosome 1q31-32. Recombinant human IL-10 is an 18.6 kDa protein of 161 amino acid residues. Interleukin 10 (IL-10) comprises interferon IFN-γ fold and exists as a symmetric homodimer. It shares structural similarity with interferon γ (IFN-γ).

Aplicação

Interleukin 10 (IL-10) human has been used in enzyme-linked immunosorbent assay.

Ações bioquímicas/fisiológicas

Interleukin-10 (IL-10) is an anti-inflammatory protein that inhibits the expression of proinflammatory cytokines such as IL-1 and tumor necrosis factor-α (TNF-α). Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. It inhibits the production of IFN-γ and antigen-specific proliferation of type-1 T helper cells (Th1). It protects the host tissue from damage during immune responses. The protein has a role in modulating viral immunity. IL-10 enhances cytokine production, natural killer cell proliferation and cytotoxicity in vitro. IL-10 is an immunosuppressive cytokine. It promotes wound repair and functions to resolve inflammation in the central nervous system. IL-10 mediates its signaling function through the interleukin receptors namely, IL-10RA and two IL-10RBs. The expression of IL-10 is regulated by signal transducer and activator of transcription 3 (STAT3) and suppressor of cytokine signaling-3 (SOCS3) ratios. Polymorphisms in the IL-10 gene is implicated in breast cancer.

forma física

Lyophilized from 20 mM Sodium Phosphate, pH 7.2.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

IL-10: A Multifunctional Cytokine in Viral Infections
Jose M. Rojas
Journal of immunology research (2017)
Infection of human macrophages and Dendritic Cells with Mycobacterium tuberculosis Induces a Differential Cytokine Gene Expression That Modulates T Cell response
Journal of Immunology (2001)
Amanda R Burmeister et al.
Frontiers in cellular neuroscience, 12, 458-458 (2018-12-14)
Resident cells of the central nervous system (CNS) play an important role in detecting insults and initiating protective or sometimes detrimental host immunity. At peripheral sites, immune responses follow a biphasic course with the rapid, but transient, production of inflammatory
Relationship between IL-10 gene -819C/T polymorphism and the risk of inflammatory bowel disease: a meta-analysis
Haien Wu
African Health Sciences, 16(3), 866-872 (2016)
P T Mehrotra et al.
Journal of immunology (Baltimore, Md. : 1950), 160(6), 2637-2644 (1998-03-24)
Human NK cell activity can be augmented in vitro by stimulation with IL-2 or IL-12, both of which also induce the production of IFN-gamma, TNF-alpha, and granulocyte-macrophage CSF by NK cells. For the first time, we demonstrate that freshly purified

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica