SRP3247

M-CSF from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinônimo(s): CSF-1, MGI-IM, Macrophage Colony Stimulating Factor
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: rat
• recombinante: expressed in E. coli
• Ensaio: ≥98% (HPLC); ≥98% (SDS-PAGE)
• forma: lyophilized
• potência: ≤5.0 ng/mL ED₅₀
• peso molecular: 18.1 kDa
• embalagem: pkg of 10 μg
• técnica(s): cell culture | mammalian: suitable
• Impurezas: <0.2 EU/μg endotoxin, tested
• cor: white to off-white
• nº de adesão UniProt: Q8JZQ0
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: rat ... CSF1(78965)

Descrição

Descrição geral

Macrophage colony stimulating factor (M-CSF) is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts.The gene encoding this protein is localized on rat chromosome 2q34. The human protein is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant rat M-CSF is a 36.2kDa homodimeric protein containing two 155 amino acid polypeptide subunits.

Ações bioquímicas/fisiológicas

Macrophage colony stimulating factor (M-CSF) is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation.

Sequência

MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP

forma física

Lyophilized from 5 mM Sodium Phosphate, pH 7.5.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable