SRP3138
PDGF-BB human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Sinônimo(s):
Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF), Platelet-Derived Growth Factor-BB
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About This Item
Código UNSPSC:
12352202
eCl@ss:
32190102
NACRES:
NA.32
Produtos recomendados
fonte biológica
human
recombinante
expressed in E. coli
Ensaio
≥98% (HPLC)
≥98% (SDS-PAGE)
Formulário
lyophilized
potência
1.0-3.0 ng/mL ED50
peso molecular
24.3 kDa
embalagem
pkg of 10 μg
técnica(s)
cell culture | mammalian: suitable
Impurezas
<0.1 EU/μg endotoxin, tested
cor
white to off-white
nº de adesão UniProt
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Informações sobre genes
human ... PDGFB(5155)
Descrição geral
PDGFs (platelet-derived growth factors) are disulfide-linked dimers consisting of two 12.0-13.5kDa polypeptide chains, designated PDGF-A and PDGF-B chains. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. The gene PDGF-B is mapped to human chromosome 22q13.1. Recombinant human PDGF-BB is a 24.3kDa disulfide-linked homodimer of two B chains (218 total amino acids).
Aplicação
PDGF-BB human has been used in the cell viability assay. It has been used as a stimulator in PDGFRα (platelet-derived growth factor receptor α)-positive HTPCs (human testicular peritubular cells).
Ações bioquímicas/fisiológicas
The three naturally occurring PDGFs (platelet-derived growth factors); PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF-B along with PDGFR-β participates in vascular maturation. It brings smooth muscle cells and pericytes to growing vessels. Heterozygous mutation in PDGF-B is associated with primary familial brain calcification.
Sequência
SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
forma física
Lyophilized from 10 mM Acetic Acid.
Reconstituição
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Skin Irrit. 2
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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