GF149

PDGF-BB, recombinant human

The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.

• Sinônimo(s): Platelet Derived Growth Factor-BB
• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

Propriedades

• fonte biológica: human
• Nível de qualidade: 100
• Ensaio: >98% (SDS-PAGE)
• fabricante/nome comercial: Chemicon^®
• técnica(s): cell culture | mammalian: suitable
• Impurezas: <0.1 ng/μg endotoxin (of PDGF-BB; 1EU/μg)
• entrada: sample type neural stem cell(s); sample type hematopoietic stem cell(s); sample type mesenchymal stem cell(s)
• nº de adesão UniProt: P01127; P31240 (mouse); Q05028 (rat)
• Condições de expedição: dry ice
• Informações sobre genes: human ... PDGFB(5155)

Descrição

Descrição geral

Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).

Product Source: E. coli

Aplicação

For most in vitro applications, PDGF-BB exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): fibroblasts, connective tissue.

The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.

Ligação

Replaces: GF018

forma física

Sterile filtered solution lyophilized from 10 mM HOAc.

The sterile filtered solution was lyophilized from 10 mM HOAc.

Armazenamento e estabilidade

The lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-BB should be stored in undiluted aliquots at -20°C for up to six months.

Nota de análise

Specific Activity: Human PDGF-BB is fully biologically active when compared to standards. The ED50 as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells is between 6-12 ng/mL.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1