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P4056

Sigma-Aldrich

Platelet-Derived Growth Factor-BB from rat

PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sinônimo(s):

PDGF-BB

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About This Item

Número MDL:
MFCD01094933
Código UNSPSC:
12352202
NACRES:
NA.77

recombinante

expressed in E. coli

Nível de qualidade

100

forma

lyophilized powder

potência

1.62 ng/mL EC50

qualidade

endotoxin tested

peso molecular

~30 kDa

embalagem

pkg of 5x50 μg
pkg of 50 μg

condição de armazenamento

avoid repeated freeze/thaw cycles

técnica(s)

cell culture | mammalian: suitable

Impurezas

≤1 EU/μg

nº de adesão UniProt

P31240

temperatura de armazenamento

−20°C

Informações sobre genes

rat ... Pdgfb(24628)

Categorias relacionadas

Descrição geral

Platelet-derived growth factor (PDGF) is a 30 kDa cationic polypeptide and consists of two disulphide linked chains A and B. PDGF is expressed as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

Aplicação

Platelet-Derived Growth Factor-BB from rat has been used as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture and adult spinal cord neurons culture.

Ações bioquímicas/fisiológicas

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF activates smooth muscle relocalization and multiplication. PDGF mediated increased smooth muscle muscle proliferation might be associated with gene regulation. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.

Outras notas

Dimer of the B chain of rat PDGF.

forma física

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 2.5 mg bovine serum albumin.

Nota de análise

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Platelet-Derived Growth Factor?BB Transactivates the Fibroblast Growth Factor Receptor to Induce Proliferation in Human Smooth Muscle Cells.
Millette E, et al.
Trends in Cardiovascular Medicine, 16.1, 25-28 (2006)
Xin Zhou et al.
Current protocols, 2(5), e416-e416 (2022-05-10)
The serine/threonine protein kinase Akt integrates diverse upstream inputs to regulate cell survival, growth, metabolism, migration, and differentiation. Mounting evidence suggests that Akt activity is differentially regulated depending on its subcellular location, which can include the plasma membrane, endomembrane, and
Skeletal muscle tissue engineering: A maturation model promoting long-term survival of myotubes, structural development of the excitation?contraction coupling apparatus and neonatal myosin heavy chain expression.
Das M, et al.
Biomaterials, 30.29, 5392-5402 (2009)
Role of platelets in the development of atherosclerosis.
Quintana L, et al.
Tissue Engineering: Part A, 15.1, 45-54 (2008)
Eileen M Bauer et al.
PloS one, 9(5), e96720-e96720 (2014-05-20)
Pulmonary hypertension (PH) is a progressive and fatal disease with no cure. Vascular remodeling in PH involves intraluminal growth of endothelial and smooth muscle cells, leading to obliterative vascular lesions. Cell growth in these lesions is quasi-neoplastic, with evidence of
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Artigos

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

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