SRP3117
MMP-1 human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Sinônimo(s):
Fibroblast collagenase
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.32
Produtos recomendados
fonte biológica
human
recombinante
expressed in E. coli
Ensaio
≥98% (HPLC)
≥98% (SDS-PAGE)
Formulário
lyophilized
peso molecular
42.7 kDa
embalagem
pkg of 10 μg
técnica(s)
cell culture | mammalian: suitable
Impurezas
<0.1 EU/μg endotoxin, tested
cor
white to off-white
nº de adesão UniProt
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Informações sobre genes
human ... MMP1(4312)
Categorias relacionadas
Descrição geral
Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. MMP-1 is expressed in various cells, including fibroblasts, keratinocytes, chondrocytes, monocytes and macrophages, hepatocytes and many tumor cells. The gene is mapped to human chromosome 11q22. Recombinant human MMP-1 is a 42.7kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain which is involved in substrate specificity and in binding TIMP-1 (tissue inhibitor of metalloproteinases 1).
Aplicação
MMP-1 (matrix metalloproteinase 1) human has been used to study effect of NF (nuclear factor)κB-mediated expression of MMP-1 in breast cancer spheroids.
Ações bioquímicas/fisiológicas
Matrix metalloproteinases (MMPs) enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-1 is a secreted collagenase with specificity toward Type I, II, III, VII, and X collagens, aggrecan, serpins, and α2-macroglobulin. It is overexpressed in lumbar intervertebral disc degeneration (IDD). It might also be associated with osteoarthritis. MMP-1 is linked with Jjuvenile idiopathic arthritis. Genetic variations in MMP-1 can affect the susceptibility and severity of this disease.
Sequência
MFVLTEGNPR WEQTHLTYRI ENYTPDLPRA DVDHAIEKAF QLWSNVTPLT FTKVSEGQAD IMISFVRGDH RDNSPFDGPG GNLAHAFQPG PGIGGDAHFD EDERWTNNFR EYNLHRVAAH ELGHSLGLSH STDIGALMYP SYTFSGDVQL AQDDIDGIQA IYGRSQNPVQ PIGPQTPKAC DSKLTFDAIT TIRGEVMFFK DRFYMRTNPF YPEVELNFIS VFWPQLPNGL EAAYEFADRD EVRFFKGNKY WAVQGQNVLH GYPKDIYSSF GFPRTVKHID AALSEENTGK TYFFVANKYW RYDEYKRSMD PGYPKMIAHD FPGIGHKVDA VFMKDGFFYF FHGTRQYKFD PKTKRILTLQ KANSWFNCRK N
forma física
Lyophilized from 20 mM TRIS, pH 9.0 + 0.1 mM Calcium Chloride + 100 mM Arginine.
Reconstituição
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Oncotarget, 6(36), 39262-39275 (2015-10-30)
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Regulation of matrix metalloproteinase expression in tumor invasion.
Westermarck J and Kahari VM
Faseb Journal, 13, 781-792 (1999)
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Abd-Allah SH
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The concentration of collagen degradation products (CDPs) may reflect the process of left ventricular remodeling (LVR). The aim of this study was to evaluate the potential diagnostic usefulness of time-resolved fluorescence spectroscopy (TRFS) in assessment of CDPs. The preliminary experiment
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