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Key Documents

SRP3058

Sigma-Aldrich

IFN-γ human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sinônimo(s):

Immune Interferon, MAF, T cell interferon, type II interferon

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

fonte biológica

human

recombinante

expressed in E. coli

Ensaio

≥98% (HPLC)
≥98% (SDS-PAGE)

forma

lyophilized

potência

5.0-10.0 ng/mL ED50

peso molecular

16.7 kDa

embalagem

pkg of 100 μg

técnica(s)

cell culture | mammalian: suitable

Impurezas

<0.1 EU/μg endotoxin, tested

cor

white to off-white

nº de adesão UniProt

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... IFNG(3458)

Descrição geral

IFN-γ (interferon γ) is an acid-labile glycosylated interferon produced by CD4 and CD8 T lymphocytes as well as activated NK (natural killer) cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC (major histocompatibility complex) proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. The gene is mapped to human chromosome 12q15. Human IFN-γ is species-specific and is biologically active only in human and primate cells. Recombinant human IFN-γ is a 16.7kDa protein containing 143 amino acid residues.

Aplicação

IFN-γ (interferon γ)-human has been used for the generation of inflammatory M1 cells from human monocytic cell line, THP1.

Ações bioquímicas/fisiológicas

IFN-γ (interferon γ) signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. It can suppress the generation of anti-inflammatory cytokines and enhance the secretion of proinflammatory cytokines. Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK (natural killer) cells and neutrophils. It is responsible for tumor rejection and is capable of killing tumor cells by autophagy or apoptosis. IFN-γ is also involved in Hepatitis E virus disease pathogenesis.

Sequência

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

forma física

Lyophilized from 10 mM Sodium Phosphate, pH 7.4.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

Perturbation of the T-cell repertoire in patients with unstable angina.
Liuzzo G
Circulation, 100, 2135-2139 (1999)
Role of TLR gene expression and cytokine profiling in the immunopathogenesis of viral hepatitis E.
Majumdar M
Journal of Clinical Virology, 73, 8-13 (2015)
Data on sulforaphane treatment mediated suppression of autoreactive, inflammatory M1 macrophages.
Pal S and Konkimalla VB
Data in Brief, 7, 1560-1564 (2016)
CD133+ liver cancer stem cells resist interferon-gamma-induced autophagy.
Li J
BMC Cancer, 16, 15-15 (2016)
Interferons and their actions.
S Pestka et al.
Annual review of biochemistry, 56, 727-777 (1987-01-01)

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