IF002

Interferon-γ Protein, Recombinant human

The Interferon-g protein (or IFN-gamma protein) is a lymphoid factor which possesses potent anti-viral activity.

• Sinônimo(s): Human Interferon, Human Interferon-γ, Recombinant Interferon-γ
• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

Propriedades

• fonte biológica: human
• Nível de qualidade: 100
• Ensaio: >98% (SDS-PAGE and HPLC)
• atividade específica: ≥2 x 10(E7) U/mg
• fabricante/nome comercial: Chemicon^®
• técnica(s): cell culture | mammalian: suitable
• Impurezas: <0.1 ng/μg endotoxin (of IFN-gamma; 1EU/μg)
• entrada: sample type hematopoietic stem cell(s); sample type mesenchymal stem cell(s)
• nº de adesão NCBI: NM_000619.2
• nº de adesão UniProt: P01579
• Condições de expedição: dry ice
• Informações sobre genes: human ... IFNG(3458)

Descrição

Descrição geral

Interferon-g (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.

Product Source: Human, expressed in E.coli

Aplicação

For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10 ng/mL. Responding cells are (partial list): Lymphocytes.

Ligação

Replaces: IF3

forma física

Sterile filtered, lyophilized from 10 mM sodium phosphate, pH 7.4.

Armazenamento e estabilidade

The lyophilized IFN-g, though stable at room temperature, is best stored desiccated at -20°C. After a quick spin, reconstitute in 1x PBS, pH 8.0 to a concentration of 0.5-1.0 mg/mL. Note: Further dilutions should be made into RPMI + 10% FCS or 5% HSA and stored at 4°C for 1 week or -20°C for future use. Reconstituted material should be stored in undiluted aliquots at -20°C for up to six months.

Nota de análise

Specific Activity: Human IFN-gamma is fully biologically active when compared to standards.The ED(50) as determined in a viral resistance assay is ≤ 0.05 ng/mL

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3