SRP3014
BDNF human
Carrier free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), suitable for cell culture
Sinônimo(s):
Brain Derived Neurotrophic Factor
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.32
Produtos recomendados
fonte biológica
human
Nível de qualidade
recombinante
expressed in E. coli
Ensaio
≥95% (SDS-PAGE)
Formulário
lyophilized
peso molecular
27.0 kDa
embalagem
pkg of 10 μg
técnica(s)
cell culture | mammalian: suitable
Impurezas
≤1.00 EU/μg endotoxin, tested
nº de adesão UniProt
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Informações sobre genes
human ... BDNF(627)
Categorias relacionadas
Descrição geral
The gene BDNF (brain derived neurotrophic factor) is mapped to human chromosome 11p14.1. BDNF is a member of the neurotrophin family of growth factors. The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0kDa homodimer of two 119 amino acid subunits linked by strong noncovalent interactions.
Ações bioquímicas/fisiológicas
Like other members of this family, BDNF (brain derived neurotrophic factor) supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR (low-affinity nerve growth factor receptor), which also binds other neurotrophins such as NGF (nerve growth factor), NT-3 (neurotrophin-3) and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB (tropomyosin-related kinase B). BDNF also plays an important role in vascular function and participates in angiogenesis. It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus.
Sequência
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
forma física
Lyophilized with no additives.
Reconstituição
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Nota de análise
The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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