G1777

Glial Cell Line-derived Neurotrophic Factor human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥98% (SDS-PAGE)

• Sinônimo(s): ATF, GDNF
• Número CE: 231-509-8
• Número MDL: MFCD00285867
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: human
• Nível de qualidade: 200
• recombinante: expressed in E. coli
• Ensaio: ≥98% (SDS-PAGE)
• Formulário: lyophilized powder
• peso molecular: ~30 kDa
• embalagem: pkg of 10 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• técnica(s): cell culture | mammalian: suitable
• Impurezas: endotoxin, tested
• cor: white
• nº de adesão UniProt: P39905
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... GDNF(2668)

Descrição

Descrição geral

Glial cell line-derived neurotrophic factor (GDNF) human gene is located on human chromosome 5p13.2.

Aplicação

Glial cell line-derived neurotrophic factor human (GDNF) has been used as a component in the neurobasal medium for neural differentiation. It has also been used for self-renewal, expansion and differentiation of spermatogonial stem cells (SSCs).

Ações bioquímicas/fisiológicas

Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share ~93% sequence homology, with strong species cross-reactivity. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using the Parkinson′s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.

Glial cell line-derived neurotrophic factor human (GDNF) acts as a morphogen in kidney development and modulates spermatogonial differentiation. Mutations in this gene may be associated with Hirschsprung′s disease, Tourette syndrome (TS) and attention deficit/ hyperactivity disorder (ADHD). It is used to treat Parkinson′s disease.

forma física

Lyophilized from a 0.2 μm filtered solution of 10 mM sodium citrate and 150 mM sodium chloride containing 0.5 mg bovine serum albumin.

Nota de análise

The biological activity of GDNF is determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)