SRE0021

Granzyme B human

recombinant, expressed in insect cells, ≥90% (SDS-PAGE)

• Sinônimo(s): Granzyme B human, Cytotoxic T-lymphocyte proteinase 2, Granzyme-2, GranzymeB, Lymphocyte protease, SECT, T-cell serine protease 1-3E
• Código UNSPSC: 12352204
• NACRES: NA.32

Propriedades

• fonte biológica: human
• Nível de qualidade: 200
• recombinante: expressed in insect cells
• Ensaio: ≥90% (SDS-PAGE)
• forma: lyophilized powder
• potência: >0.004 units/mg
• atividade específica: ≥40 units/μg
• embalagem: pkg of 5 μg
• nº de adesão UniProt: P10144
• Condições de expedição: wet ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... GZMB(3002)

Descrição

Descrição geral

Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B.

Ações bioquímicas/fisiológicas

Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis. It induces apoptosis in caspase-dependent and caspase-independent manner. After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell.

Definição da unidade

One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl₂.

forma física

Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable