SML1665
Nocodazole
≥98% (HPLC), DMSO solution, microtubule inhibitor
Sinônimo(s):
Methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate, Nocidazole, Nocodazolum, Oncodazole, R 17934
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Fórmula empírica (Notação de Hill):
C14H11N3O3S
Número CAS:
Peso molecular:
301.32
Código UNSPSC:
12352200
NACRES:
NA.77
Produtos recomendados
Nome do produto
Nocodazole Ready Made Solution, 5 mg/mL, DMSO solution
fonte biológica
synthetic
Nível de qualidade
Formulário
DMSO solution
concentração
5 mg/mL
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
cadeia de caracteres SMILES
[s]1c(ccc1)C(=O)c2cc3nc([nH]c3cc2)NC(=O)OC
InChI
1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)
chave InChI
KYRVNWMVYQXFEU-UHFFFAOYSA-N
Ações bioquímicas/fisiológicas
Nocodazole is an anticancer drug that has been shown to interfere with the structure and function of microtubules in interphase and mitotic cells.
Nocodazole is an anticancer drug that has been shown to interfere with the structure and function of microtubules in interphase and mitotic cells. Malignant cells may be more susceptible to the antimicrotubular effect of nocodazole than nonmalignant cells. Mammalian cells cultured in vitro can be treated with 0.04-10 μg/mL doses for cell synchronization experiments. Prolonged arrest of cells in mitosis due to nocodazole treatment typically results in cell death by apoptosis. Higher concentrations could not be used because of insolubility. High specificity of action may explain low toxicity to bone marrow cells and lack of neurotoxicity. Nocodazole is thought to bind directly to tubulin causing conformational changes resulting in increased exposure of some sulfhydryl and possibly tyrosine residues. Nocodazole′s apparent synergism with cytosine arabinofuranoside has been demonstrated on L1210 leukemic cells.
Nota de preparo
Nocodazole forms precipitates in frozen solution, therefore sonication should be applied after thaw to homogenize the solution. It is recommended to sonicate for 1-3 minutes post thawing to remove precipitates that might appear due to freezing.
For cell culture work, dilute Nocodazole ready-made solution (5 mg/mL DMSO) in the culture media, diluted solution is stable for at least 7 days in biological. Mammalian cells cultured in vitro can be treated with 0.04-10 μg/mL doses.
For cell culture work, dilute Nocodazole ready-made solution (5 mg/mL DMSO) in the culture media, diluted solution is stable for at least 7 days in biological. Mammalian cells cultured in vitro can be treated with 0.04-10 μg/mL doses.
Armazenamento e estabilidade
The product is stable for 2 years at -20C.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
188.6 °F - closed cup
Ponto de fulgor (°C)
87 °C - closed cup
Escolha uma das versões mais recentes:
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Karin Purshouse et al.
eLife, 11 (2022-12-09)
Extrachromosomal DNA (ecDNA) are frequently observed in human cancers and are responsible for high levels of oncogene expression. In glioblastoma (GBM), ecDNA copy number correlates with poor prognosis. It is hypothesized that their copy number, size, and chromatin accessibility facilitate
Pierre-Olivier Estève et al.
Communications biology, 5(1), 1292-1292 (2022-11-27)
In mammalian cells, SET8 mediated Histone H4 Lys 20 monomethylation (H4K20me1) has been implicated in regulating mitotic condensation, DNA replication, DNA damage response, and gene expression. Here we show SET8, the only known enzyme for H4K20me1 is post-translationally poly ADP-ribosylated
M Parant et al.
Ecotoxicology and environmental safety, 182, 109385-109385 (2019-07-02)
The present study was the first approach conducted under environmental concentrations of Gd-DOTA and Gd-DTPA-BMA to assess cellular impacts of these compounds. Gd-DOTA (Gadoteric acid) is one of the most stable contrast agent, currently used as Dotarem® formulation during Magnetic
Katarina T Miteva et al.
The Journal of cell biology, 218(7), 2232-2246 (2019-05-17)
Endothelial cells selectively release cargo stored in Weibel-Palade bodies (WPBs) to regulate vascular function, but the underlying mechanisms are poorly understood. Here we show that histamine evokes the release of the proinflammatory ligand, P-selectin, while diverting WPBs carrying non-inflammatory cargo
Sara De Vincentiis et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(26), 4997-5007 (2020-05-24)
Stretch-growth has been defined as a process that extends axons via the application of mechanical forces. In the present article, we used a protocol based on magnetic nanoparticles (NPs) for labeling the entire axon tract of hippocampal neurons, and an
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica