SAE0023

Alanine Racemase from Bacillus stearothermophilus

buffered aqueous solution, ≥10 IU/mg solid

• Sinônimo(s): Alanine Racemase from Bacillus stearothermophilus
• Número CAS: 9024-06-0
• Número da licença da enzima: 5.1.1.1 (BRENDA, IUBMB)
• Número MDL: MFCD03454300
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• fonte biológica: Bacillus sp. (B. stearothermophilus)
• Nível de qualidade: 200
• Formulário: buffered aqueous solution
• atividade específica: ≥10 IU/mg solid
• peso molecular: M_r 78 kDa (2 subunits 39 kDa each)
• temperatura de armazenamento: −20°C

Descrição

Aplicação

Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-¹⁴C]alanine to a racemic mixture of L/D- [¹⁴C]alanine †.

Ações bioquímicas/fisiológicas

Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†

Definição da unidade

One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.

forma física

Aqueous solution containing 10 mM potassium phosphate

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable