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Key Documents

SAB4503781

Sigma-Aldrich

Anti-phospho-Smad3 (pSer425) antibody produced in rabbit

affinity isolated antibody

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

peso molecular

antigen 48 kDa

reatividade de espécies

human, rat, mouse

concentração

~1 mg/mL

técnica(s)

ELISA: 1:40000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

phosphorylation (pSer425)

Informações sobre genes

human ... SMAD3(4088)

Descrição geral

SMAD3 (SMAD family member 3) is located on human chromosome 15q22. Smad 3 belongs to the mothers against Dpp (MAD) related family of proteins.

Imunogênio

The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425.

Immunogen Range: 376-425

Aplicação

Anti-phospho-Smad3 (pSer425) antibody has been used in immunoprecipitation and immunoblotting.

Ações bioquímicas/fisiológicas

SMAD (homologues of the Drosophila protein, mothers against decapentaplegic (Mad) and the Caenorhabditis elegans protein Sma) proteins play an important role in the intracellular signalling of transforming growth factor β (TGFβ). Reduction in the phosphorylation level of Smad3 helps autophagy to control the endothelial-mesenchymal transition. In human arterial smooth muscle cells, suppressing siRNA of SMAD3 improves the viability of cells.

Características e benefícios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

forma física

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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XBP 1-Deficiency Abrogates Neointimal Lesion of Injured Vessels Via Cross Talk With the PDGF Signaling
Zeng L, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 35(10), 2134-2144 (2015)
Mariusz Popek et al.
International journal of molecular sciences, 23(3) (2022-02-16)
Decreased platelet count represents a feature of acute liver failure (ALF) pathogenesis. Platelets are the reservoir of transforming growth factor 1 (TGF-β1), a multipotent cytokine involved in the maintenance of, i.a., central nervous system homeostasis. Here, we analyzed the effect
Direct binding of Smad3 and Smad4 to critical TGF beta-inducible elements in the promoter of human plasminogen activator inhibitor-type 1 gene
Dennler S, et al.
The Embo Journal, 17(11), 3091-3100 (1998)
Autophagy regulates Endothelial-Mesenchymal transition by decreasing the phosphorylation level of Smad3
Wang J, et al.
Biochemical and Biophysical Research Communications, 487(3), 740-747 (2017)
TbetaRI phosphorylation of Smad2 on Ser465 and Ser467 is required for Smad2-Smad4 complex formation and signaling
Abdollah S, et al.
The Journal of Biological Chemistry, 272(44), 27678-27685 (1997)

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