SAB4200846
Anti-LC3C antibody, Mouse monoclonal
clone LC3C-37, purified from hybridoma cell culture, affinity isolated antibody
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
Produtos recomendados
forma do anticorpo
affinity isolated antibody
purified from hybridoma cell culture
tipo de produto de anticorpo
primary antibodies
clone
LC3C-37, monoclonal
reatividade de espécies
human
embalagem
antibody small pack of 25 μL
concentração
~1 mg/mL
técnica(s)
immunoblotting: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human LC3C.
immunofluorescence: 2.5-5 μg/mL using human HeLa cells treated with Bafilomycin A1 for autophagosomes accumulation
Isotipo
IgG1
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... MAP1LC3C(440738)
Descrição geral
At least 16 genes encoding for autophagy (ATG) related proteins that are required for autophagosome formation were identified in yeast by genetic screens. For many of these genes, related homologs have been identified in mammals.6 The Microtubule-associated protein light chain 3 (MAP1LC3), the mammalian homolog of yeast Atg8/Apg8/Aut7, is essential for the formation of autophagosomes.7 In addition, three human orthologs of yeast Atg8, named LC3A (MAP1LC3A), LC3B (MAP1LC3B) and LC3C (MAP1LC3C), were identified. These three human isoforms show different expression patterns in human tissues.8The LC3 protein exists in two forms, cytoplasmic LC3-I (18 kDa) and autophagosome membrane associated LC3-II (16 kDa). LC3-II is the result of LC3-I cleavage at the C-terminal Glycine by cysteine protease Atg4, which is further modified by the mammalian E1- and E2-like enzymes: Atg7 and Atg3.7 The levels of LC3-II protein correlates with the extent of autophagosome formation.3 Thus, LC3C can be used as an autophagosomal and mitophagy marker.3,7,9-11
Especificidade
Monoclonal Anti-LC3C antibody specifically human LC3C-I and/or LC3C-II and dose not cross react with human LC3A or LC3B.
Imunogênio
synthetic peptide corresponding to the N-terminal region of human LC3C, conjugated to KLH
Aplicação
The antibody may be used in various immunochemical techniques including Immunoblotting and Immunofluorescence. Detection of the LC3C band by Immunoblotting is specifically inhibited by the immunogen.
Ações bioquímicas/fisiológicas
Macroautophagy, usually referred to as an autophagy, is a major pathway for bulk degradation of cytoplasmic constituents and organelles. In this process, portions of the cytoplasm are sequestered into double membrane vesicles, called autophagosomes, and subsequently delivered to the lysosome for degradation and recycling.1,2 Although autophagy is a constitutive cellular event, it is enhanced under certain conditions such as starvation, hormonal stimulation and drug treatments.3 Autophagy plays an essential role in cellular differentiation, cell death and aging and is required for normal turnover of cellular components during starvation. Defective autophagy process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections.4,5 Autophagy pathway is evolutionary conserved in all eukaryotic cells.
forma física
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Armazenamento e estabilidade
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Exoneração de responsabilidade
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
nwg
Escolha uma das versões mais recentes:
Certificados de análise (COA)
Lot/Batch Number
Não está vendo a versão correta?
Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
A unified nomenclature for yeast autophagy-related genes.
Daniel J Klionsky et al.
Developmental cell, 5(4), 539-545 (2003-10-11)
D J Klionsky et al.
Science (New York, N.Y.), 290(5497), 1717-1721 (2000-12-02)
Macroautophagy is a dynamic process involving the rearrangement of subcellular membranes to sequester cytoplasm and organelles for delivery to the lysosome or vacuole where the sequestered cargo is degraded and recycled. This process takes place in all eukaryotic cells. It
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.
Y Kabeya et al.
The EMBO journal, 19(21), 5720-5728 (2000-11-04)
Little is known about the protein constituents of autophagosome membranes in mammalian cells. Here we demonstrate that the rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of Apg8p essential for autophagy in yeast, is associated to the autophagosome
Alexandra Stolz et al.
The EMBO journal, 36(4), 549-564 (2016-12-29)
Autophagy is a cellular surveillance pathway that balances metabolic and energy resources and transports specific cargos, including damaged mitochondria, other broken organelles, or pathogens for degradation to the lysosome. Central components of autophagosomal biogenesis are six members of the LC3
Helena J Maier et al.
Autophagy, 9(4), 496-509 (2013-01-19)
Autophagy is a highly conserved cellular response to starvation that leads to the degradation of organelles and long-lived proteins in lysosomes and is important for cellular homeostasis, tissue development and as a defense against aggregated proteins, damaged organelles and infectious
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica