SAB4200746

Anti-α-Fetoprotein (AFP) antibody, Mouse monoclonal

clone C3, purified from hybridoma cell culture

• Sinônimo(s): Anti-AFP, Anti-Alpha-1-fetoprotein, Anti-Alpha-fetoglobulin
• Código UNSPSC: 12352203
• NACRES: NA.46

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: C3, monoclonal
• Formulário: buffered aqueous solution
• peso molecular: ~74 kDa
• reatividade de espécies: human, canine, porcine
• concentração: ~1.0 mg/mL
• técnica(s): ELISA: suitable; dot blot: suitable; immunoblotting: 2-4 μg/mL using liver hepatocellular carcinoma HepG2 cell line extract; immunofluorescence: suitable; immunohistochemistry: 2-4 μg/mL using formalin-fixed, paraffin-embedded human fetal liver sections
• Isotipo: IgG2a
• nº de adesão UniProt: P02771
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... AFP(174)

Descrição

Descrição geral

The gene encoding α-fetoprotein (AFP) is localized on human chromosome 4. It encodes a plasma glycoprotein that has a molecular mass of 70kDa. It is synthesized along with serum albumin during the development of embryonic yolk sac and the liver.

Imunogênio

Purified human α-fetoprotein

Ações bioquímicas/fisiológicas

α-fetoprotein (AFP) is involved in the regulation of fatty acids in both fetal and proliferating adult liver cells. Its expression is found to be increased in acute liver injuries, indicating active liver regeneration. AFP has been associated with fatty liver disease (FLD), a disease that may lead to cirrhosis and hepatocellular carcinoma. It serves as a tumor marker for HCC (hepatocellular carcinoma).

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Outras notas

In order to obtain best results in different techniques and preparations, we recommend determining optimal working concentration by titration test.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable