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SAB4200141

Sigma-Aldrich

Anti-Rictor antibody produced in rabbit

~1-2 μg/mL, affinity isolated antibody

Sinônimo(s):

ANTI-Pianissimo, ANTI-Rapamycin-insensitive companion of mTOR, ANTI-TORC2-specific protein AVO3, Anti-RPTOR independent companion of MTOR, complex 2, AVO3

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.44

fonte biológica

rabbit

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

Formulário

buffered aqueous solution

peso molecular

~190 kDa

reatividade de espécies

human, rat, mouse

embalagem

antibody small pack of 25 μL

concentração

~1-2 μg/mL

técnica(s)

immunohistochemistry: 5-10 μg/mL using a working concentration of 5-10 ug/mL is recommended using heat-retrieved formalin-fixed, paraffin-embedded rat testis sections and biotin / ExtrAvidin Peroxidase staining system.
immunoprecipitation (IP): 5-10 μg using a working amount of 5-10 μg is recommended using lysates of human HeLa cells.
western blot: 1-2 μg/mL using a working concentration of 1-2 μg/mL is recommended using whole extracts of mouse C2C12 cells.

nº de adesão UniProt

Q6R327

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... RICTOR(253260)
mouse ... rictor(78757)
rat ... rictor(310131)

Descrição geral

Rictor (rapamycin-insensitive companion of mTOR) is a subunit of the mammalian target of rapamycin complex 2 (mTORC2). The mTORC2 complex contains mTOR, mLST8, mSIN1, deptor, protor-1 and rictor. mTOR substrate that regulates cell size.
m-TOR is a conserved kinase that primarily regulates cell growth. Rictor is a rapamycin-insensitive companion of m-TOR and regulates the phosphorylation of Akt/PKB. Rictor also phosphorylates PKCα and actin cytoskeleton .

Especificidade

Anti-Rictor antibody is specific for human, mouse and rat rictor. In immunoblotting, detection of the rictor band (approx. 190 kDa) is specifically inhibited by the immunizing peptide.

Imunogênio

The corresponding sequence is identical in mouse and rat rictor.

Aplicação

Anti-Rictor antibody is suitable for use in immunohistochemistry (5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded rat testis sections and biotin/ExtrAvidin Peroxidase staining system). The product may also be used for immunoprecipitation (5-10 μg using lysates of human HeLa cells), and immunoblot (1-2 μg/mL is using whole extracts of mouse C2C12 cells).

Ações bioquímicas/fisiológicas

Rictor (rapamycin-insensitive companion of mTOR) regulates cell survival and cytoskeletal organization through the regulation of Akt/ protein kinase B (PKB) and protein kinase C alpha (PKCα).

forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
040M4799

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Visite a Biblioteca de Documentos

mTORC2 activity is elevated in gliomas and promotes growth and cell motility via overexpression of rictor
Masri J, et al.
Cancer research, 67(24), 11712-11720 (2007)
D D Sarbassov et al.
Science (New York, N.Y.), 307(5712), 1098-1101 (2005-02-19)
Deregulation of Akt/protein kinase B (PKB) is implicated in the pathogenesis of cancer and diabetes. Akt/PKB activation requires the phosphorylation of Thr308 in the activation loop by the phosphoinositide-dependent kinase 1 (PDK1) and Ser473 within the carboxyl-terminal hydrophobic motif by
Targeting mTORC2 inhibits colon cancer cell proliferation in vitro and tumor formation in vivo
Roulin D, et al.
Molecular Cancer, 9(1), 57-57 (2010)
D D Sarbassov et al.
Current biology : CB, 14(14), 1296-1302 (2004-07-23)
The mammalian TOR (mTOR) pathway integrates nutrient- and growth factor-derived signals to regulate growth, the process whereby cells accumulate mass and increase in size. mTOR is a large protein kinase and the target of rapamycin, an immunosuppressant that also blocks
Estela Jacinto et al.
Nature cell biology, 6(11), 1122-1128 (2004-10-07)
The target of rapamycin (TOR) is a highly conserved protein kinase and a central controller of cell growth. In budding yeast, TOR is found in structurally and functionally distinct protein complexes: TORC1 and TORC2. A mammalian counterpart of TORC1 (mTORC1)
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