S8884

Safranin O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

• Sinônimo(s): Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
• Fórmula empírica (Notação de Hill): C₂₀H₁₉ClN₄
• Número CAS: 477-73-6
• Peso molecular: 350.84
• Número do índice de cores: 50240
• Beilstein: 3924099
• Número CE: 207-518-8
• Número MDL: MFCD00011759
• Código UNSPSC: 12171500
• ID de substância PubChem: 24899820
• NACRES: NA.47

Propriedades

• Nome do produto: Safranin O, certified by the Biological Stain Commission
• grau: certified by the Biological Stain Commission
• Formulário: powder
• composição: Dye content, ≥80%
• técnica(s): microbe id | staining: suitable
• cor: green to very dark green
• solubilidade: H₂O: 1 mg/mL
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: [Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
• InChI: 1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
• chave InChI: OARRHUQTFTUEOS-UHFFFAOYSA-N

Descrição

Descrição geral

Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.

Aplicação

Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.

Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.

Adequação

Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.

Informações de segurança

• Pictogramas: GHS05
• Palavra indicadora: Danger
• Frases de perigo: H318
• Declarações de precaução: P280 - P305 + P351 + P338
• Classificações de perigo: Eye Dam. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves