1.09217

Gram′s safranine solution

for the Gram staining method

• Código UNSPSC: 41116130
• NACRES: NA.74

Propriedades

• Nível de qualidade: 400
• Formulário: liquid
• IVD: for in vitro diagnostic use
• técnica(s): microbe id | staining: suitable
• temperatura de transição: flash point 49 °C
• densidade: 0.98 g/cm³ at 20 °C
• aplicação(ões): clinical testing; diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: 15-25°C

Descrição

Descrição geral

This ready-to-use Gram′s safranine solution-for the Gram staining method, is used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram′s crystal violet solution (Product number 1.09218) - an aniline dye - in the first step. After the treatment with iodinesolution (Lugol′s solution stabilized with PVP, Product number 1.00567), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, 1.10218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram′s safranin solution and appear pink to red.
A bottle with 500 ml Gram′s safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Product number 1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P210 - P233 - P240 - P241 - P242 - P243
• Classificações de perigo: Flam. Liq. 3
• Código de classe de armazenamento: 3 - Flammable liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): 120.2 °F
• Ponto de fulgor (°C): 49 °C