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Key Documents

84120

Sigma-Aldrich

Safranin O

for microscopy (Bact., Bot., Hist.), indicator (pH 0.3-1.0)

Sinônimo(s):

Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A

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About This Item

Fórmula empírica (Notação de Hill):
C20H19ClN4
Número CAS:
Peso molecular:
350.84
Número do índice de cores:
50240
Beilstein:
3924099
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:
NACRES:
NA.47

grau

for microscopy (Bact., Bot., Hist.)
indicator (pH 0.3-1.0)

Nível de qualidade

forma

powder or crystals

técnica(s)

titration: suitable

pH

10 (20 °C, 10 g/L)

solubilidade

methanol: 0.01 g/10 mL, red

εmax

≥1000 at 525-535 nm in 50% ethanol

aplicação(ões)

diagnostic assay manufacturing
hematology
histology
microbiology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

chave InChI

OARRHUQTFTUEOS-UHFFFAOYSA-N

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Descrição geral

Safranin O, also known as Basic Red 2, is a basic, cationic, and metachromatic azine dye used as a biological stain. The characteristic azine chromophore contains the phenazine, a planar, tricyclic dibenzopyrazine, which consists of two non-planar benzene rings linked by two nitrogen atoms. A delocalized positive charge, an alternation of aromatic and quinonoid structures, and diazotizable amine groups constitute the chromophoric system. c-Methylation makes this dye of moderate overall size. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. The dye is commercially available as a chloride.

Aplicação

  • Safranin O is widely employed as a microbiological stain, especially as a counterstain in Gram staining to distinguish Gram-positive from Gram-negative micro-organisms, and for staining bacterial spores.
  • It is used to demonstrate glycosaminoglycans in human and animal histological specimens and nuclei.
  • It is a component of Benda′s polychrome stain.
  • It is a component of various polychromes and lignin stains for botanical staining applications.
  • It has also been applied for the demonstration of mast cells in cytospin preparations and staining surgical frozen sections.

Pictogramas

Corrosion

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

Classificações de perigo

Eye Dam. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Gloves


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