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S5897

Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, fine

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About This Item

Número CAS:
9004-54-0
Número MDL:
MFCD00132771
Código UNSPSC:
23151817
NACRES:
NA.52

grau

DNA grade
for molecular biology

Nível de qualidade

200

linha de produto

BioReagent

forma

beads

técnica(s)

DNA purification: suitable

matriz

cross-linked dextran

inchaço

1 g swells to 9-11 mL

diâmetro da esfera

20-80 μm

tamanho de poro

exclusion limit (20 bp dsDNA (rA)20)

pH

2-10

atividade externa

DNase, RNase, none detected

temperatura de armazenamento

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

chave InChI

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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Descrição geral

Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

S5897-100G′s updated product number is GE17-0573-02

Aplicação

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.

Características e benefícios

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Informações legais

Sephadex is a registered trademark of Cytiva

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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